University of Virginia Biology Dept. DNA Sequencing Facility

Welcome to the University of Virginia Biology Department's Genome Sequencing Core!
	The Department of Biology at the University of Virginia has recently acquired a Roche 454 FLX Genome Sequencing System. This state-of-the-art instrument serves as the cornerstone of our growing Comparative Genomics core. We are pleased to offer a range of genome sequencing services to individuals within and outside of our academic community. Process Overview: Please visit this link for an comprehensive description of the sequencing process Briefly, the sample workflow is as follows: Customers submit a DNA sample that meets all quality control benchmarks set forth by the Genomics Core A library is created via shearing and then ligation of process-specific adaptor molecules to the customer's sample This library undergoes a concentration titration in an oil-in-water emulsion PCR reaction (emPCR) to determine the optimum amount of template library needed to produce enough clonal DNA for sequencing A production emPCR is carried out to amplify sufficient DNA copies for sequencing The library is deposited onto a sequencing plate and the sequencing run is carried out Run data is stored for XXX hrs on the Core clusters, during which time it can be downloaded via FTP. The Core can also provide a hard copy customer data at at-cost. 454 Sequencing Highlights: 400-600 megabase (Mb) with greater-than 1 million high-quality reads per run 99% accuracy at base 400, > 99% at preceding bases (q20 = 400) Unidirectional sequencing pass 400 bases (average) with Titanium chemistry Ability to process dsDNA from genomic, organelle, BAC, YAC P1 Multiplex Identifier Taqs (MIDs) allow for sample pooling after library construction to reduce user expense and maximize data output *Applications: Prokaryotic/eukaryotic whole genome sequencing Resequencing/deep sequencing SNP detection Metagenomics Gene discovery ChIP-seq/Methylation/Epigenetics Current Services Offered: Standard (shotgun) library preparation 3 - 8kb Paired-end library preparation Sequencing from any library source (e.g. transciptome, amplicon, etc.) Cost of Service: Service prices vary depending upon the user's affiliation (academic, non-profit and commercial) For a comprehensive list of applications, please visit Roche's website *Breadth of services will expand concomitantly with Roche product release.