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  Speciation  Lab - 

Phytophthora pre-mating isolation

Reproductive isolation between lineages and its applied significance.

Biology of Phytophthora
Experiment 1 - Prezygotic isolating mechanisms
Crosses
Data Collection
Instructor Hints

potato blight tomato blight
Potato (left) and tomato (right) blight
Pictures from David Shaw's page: http://biology.bangor.ac.uk/~bss081/


Sexual Reproduction in the genus Phytophthora.


Figure 2 - Life Cycle of Phytophthora
 


life cycle

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Experiment to investigate pre-zygotic isolating mechanisms

phyto phylog

Source: Cooke, D. E. L. and Duncan, J. M. 1997. Mycological-Research 101:667-677

Crossing design


                                                           
A2 P. infestans (Mexico [6636 or 510])
A1 P. infestans (Mexico)

A1 P. infestans (The Netherlands)

A1 P. mirabilis (Mexico)

A1 P. parasitica (North Carolina)

A1 P. drechsleri (North Carolina)

A1 P. cinnamomi (North Carolina)



Preparing mating crosses:

phyto plate

NEXT TIME: We will use microscopes to quantify the number of oogonia and antheridia produced in the
various crosses.

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Reproductive Isolation in Phytophthora - Data Collection


step 6 pict


sporangia


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