Associated Movies for
Mundy DI, Machleidt T, Ying Y-S, Anderson RGW, and Bloom GS. 2002. Control of Caveolar Membrane Traffic by
Microtubules and the Actin Cytoskeleton. JOURNAL OF CELL SCIENCE 115: 4327-4339.

Click for Movie 1 Control CHO cell stably expressing caveolin-1-GFP. The video shows several tubules, both branched and unbranched, that are rapidly elongating and shortening. The still associated with this video is shown in Fig. 4B. Movie: 100 frames; 3.2 seconds per frame. Playback: 15 fps.
Click for Movie 2 Control CHO cells stably expressing caveolin-1-GFP. The video shows typical control cells exhibiting both short-range saltatory movements and the long-range microtubule-based movements. The video contains some excellent examples of long-range movements in both directions and also shows how convoluted some of these paths are. The still associated with this video is shown in Fig. 4C. Movie: 220 frames; 1.6 seconds per frame. Playback: 15 fps.
Click for Movie 3 Control CHO cell stably expressing caveolin-1-GFP. In this experiment the image was cropped and scaled using NIH Image to be two times larger than the original in order to show the microtubule-based movements in greater detail. There are four motile structures, two of which turn and curve sharply back as they trace the microtubule track. The still associated with this video is shown in Fig. 4C. Movie: 59 frames; 1.6 seconds per frame. Playback: 15 fps.
Click for Movie 4 Caveosome photobleaching in a CHO cell stably expressing caveolin-1-GFP. The peri-centrosomal structure labeled by caveolin-1-GFP of one cell was bleached to determine whether this region of the cell was actively exchanging with caveolin-1-GFP at the plasma membrane. Recovery was apparent within 1 minute. The stills associated with this video are shown in Fig. 5. Movie: 243 frames; 1.6 seconds per frame. Playback: 15 fps.
Click for Movie 5 Effects of nocodazole in CHO cells stably expressing caveolin-1-GFP. CHO cells were treated with nocodazole before imaging. All long-range movements disappeared. Note that much of the caveolin-1-GFP has become aligned along the cell surface. The still associated with this video is shown in Fig. 6A. Movie: 220 frames; 1.6 seconds per frame. Playback: 15 fps.
Click for Movie 6 Effects of nocodazole and latrunculin in CHO cells stably expressing caveolin-1-GFP. CHO cells were pretreated with nocodazole before the addition of latrunculin, and imaging was begun immediately. Disruption of the both the actin cytoskeleton and microtubules blocked all movements. Starting at frame number 141 the focal plane was moved to closer to the middle of the cell to show that movements within the cell have also disappeared. The still associated with this video is shown in Fig. 6C. Movie: 220 frames; 1.6 seconds per frame. Playback: 15 fps.
Click for Movie 7 Latrucnculin effects in CHO cells stably expressing caveolin-1-GFP. CHO cells were imaged immediately after the addition of latrunculin. The collection and playback times are the same as those shown for the control videos shown in Movies 2 and 3. Note that the number and speed of the movements have increased dramatically. The still associated with this video is shown in Fig. 10A. Movie: 220 frames; 1.6 second per frame. Playback: 15 fps.
Click for Movie 8 Latrucnculin effects in CHO cells stably expressing caveolin-1-GFP. These cells were treated with latrunculin and imaged continuously for approximately 20 minutes. This video shows some good examples of caveolin-1-GFP positive structures moving both into and out of the long processes that are keeping the cells attached to the substratum. The still associated with this video is shown in Fig. 10A. Movie: shows 200 of 400 frames; 1.6 second per frame. Playback: 15 fps.