Associated movies for : King ME, Kan H-M, Baas P, Erisir A, Glabe CG, and Bloom GS. 2006. Tau-Dependent Microtubule Disassembly Induced by Pre-fibrillar ß-Amyloid. Journal of Cell Biology 175: 541-546.

 

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Video 1 (corresponds to Figure 1A). Microtubules disassemble in tau-expressing CV-1 cells exposed to pre-fibrillar Aβ42. CV-1 cells transfected with tau-CFP and YFP-tubulin were treated with 1 µM pre-fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules disassembled. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 2 Microtubules disassemble in tau-expressing CV-1 cells exposed to pre-fibrillar Aβ40. CV-1 cells transfected with tau-CFP and YFP-tubulin were treated with 3 µM pre-fibrillar Aβ40, and imaged by time lapse fluorescence microscopy. Note that microtubules disassembled. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 3 (corresponds to Figure 1B).  Tau is required for pre-fibrillar Aβ42 to induce microtubule disassembly. CV-1 cells transfected with YFP-tubulin were treated with 1 µM pre-fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules did not disassemble. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 4 (corresponds to Figure 1C). Fibrillar Aβ42 does not induce microtubule disassembly in tau-expressing cells. CV-1 cells transfected with tau-CFP and YFP-tubulin were treated with 1 µM fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules did not disassemble. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 5 (corresponds to Figure 4A, top panel). Pre-fibrillar Aβ42 does not induce microtubule disassembly in MAP2c-expressing cells. CV-1 cells transfected with GFP-MAP2c were treated with 1 µM fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules did not disassemble. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 6 (corresponds to Figure 4A, middle panel). Pre-fibrillar Aβ42 does not induce microtubule disassembly in cells expressing MAP2c chimera. CV-1 cells transfected with GFP-MAP2c chimera were treated with 1 µM fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules did not disassemble. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 7 (corresponds to Figure 4A, bottom panel). Pre-fibrillar Aβ42 induces microtubule disassembly in cells expressing tau chimera. CV-1 cells transfected with GFP-tau chimera were treated with 1 µM fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules disassembled. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.

 

Video 8 (corresponds to Figure 4B). Pre-fibrillar Aβ42 induces microtubule disassembly in cells expressing the N-terminal tau arm. CV-1 cells transfected with tau arm-CFP plus YFP-tubulin were treated with 1 µM fibrillar Aβ42, and imaged by time lapse fluorescence microscopy. Note that microtubules disassembled even though tau arm-CFP was not targetted to microtubules. The capture rate was 1 frame/minute, and the playback rate is 12 frames/second.