Surface Mesoderm Morphogenesis Project


 

David Shook is working to understand the morphogenesis of mesoderm that originates superficially in the pre-gastrula embryo, in a number of different amphibian species. We are investigating this on a number of levels, including characterizing the amount, timing and location of superficial mesoderm ingression, characterizing the specification of this tissue, both at the level of fate mapping, and at the level of the molecular patterning markers expressed, and characterizing the cellular behavior of this tissue, in terms of adhesion molecules and markers of epithelial vs. mesenchymal character.

 

Section of an embryo that was surface biotinylated at stage 9; label is found in the deep layers at stage 17-18, in the somites and notochord.