The anterior cruciate ligament (ACL) is the most commonly injured ligament of the knee, due to inherently poor healing potential and limited vascularization ACL ruptures do not heal and surgical replacement is often required. Our laboratory has produced a cell-seeded, degradable, three-dimensional (3-D) scaffold for ACL replacement and regeneration.  Three-dimensional braiding techniques have been used to create a scaffold with optimized pore size for cell proliferation and tissue ingrowth, resistance to wear and rupture, and mechanical properties comparable to natural ACL.  Presently, we are optimizing this structure to improve the biomechanical properties, adjust degradation time, and enhance the cell proliferation and tissue regeneration  abilities of the implant.  These changes will result in an implant that berhaves more like natural ligament and enhances regeneration without rupturing after implantation.