Lab Protocols
Changing CO2 tanks on incubators
Thawing mammalian cells
Freezing mammalian cells
Heat inactivation of FBS
Lysis buffer recipe
Lysing adherent mammalian cells
IP lysis buffer recipe
Immunoprecipitation protocol
MicroBCA Protein Assay
NuPage Western Blot Protocol
Trans-Blot Turbo Semidry Blotting
Wet tank transfer protocol
Lentivirus preparation
Lentivirus transfection worksheet, 3rd generation packaging
Lentivirus transfection worksheet, 2nd generation packaging
Retrovirus preparation using Phoenix cells
Retrovirus transfection worksheet
Coverslip immunofluorescence
Growing cell spheroids in collagen
Soft agar colony formation assay
Wound healing assay
Ethanol fixation for cell cycle analysis
Media recipe for mouse PDAC cell lines
Subculturing glioma stem cells
Transforming competent bacterial cells
Making LB agar plates
Growing bacterial cultures
Concentrating DNA by precipitation in ethanol
Reconstituting DNA from filter paper
Creating a heatmap in MeV
R Studio code for PCA, Pearson correlations, & heatmaps
Protocol-relevant links
Typical
concentrations of antibiotic selection agents
Lab equipment manuals & links to tutorials
Thermo
TSU -80 freezerThermo Locator 6 Plus Cryogenic Bank
Thermo Forma i160 SteriCycle CO2 incubators
Eppendorf 5430/5430R Centrifuges
Heraeus Megaufuge 16
TX-400 Rotor
Mettler Toledo ML54T Balance
Millipore Synergy
BioRad Trans-Blot Turbo Blotting System
LI-COR Odyssey CLx Operator's Manual
Odyssey CLx Application Protocols Manual
Tech Note -- Near-Infrared (NIR) Western Blot Detection
Good Westerns Gone Bad for NIR -- Tips to Make Your NIR Western Blot Great
Good Normalization Gone Bad
Image Studio Online Tutorial
Image Studio Lite Online Tutorial
Basic Concepts in Microscopy (Zeiss)
Chroma optical filter sets (Axiovert 40 CFL)
DAPI/Hoechst; AF488/FITC; TRITC/Rhodamine
Zeiss optical filter sets (AxioObserver Z1)
Available filter sets & objectives
DAPI; CFP; AF488; YFP; AF546; AF594; AF647
Databases
cBioPortal for
Cancer Genomics
PROGeneV2
Prognostic Database
PRECOG - Prediction
of Clinical Outcomes from Genomic Profiles
CellMiner
(NCI)
COSMIC
Catalogue of somatic mutations in cancer
Genomics of Drug Sensitivity in Cancer Project
Other links
UVA MolMartUVA Environmental Health & Safety