Figure 1. Dose response curve demonstrating controlled expression in a cell line coexpressing tTS and rtTA. HR5 cells, which constitutively express rtTA, were transiently transfected with a plasmid expressing tTS and a control vector expressing luciferase downstream of the TRE. Cells were cultured in the indicated levels of Dox. After 24 hr,cells were harvested, and assayed for luciferase activity. SD = silencing domain. AD = activation domain. Data provided courtesy of S. Freundlieb, UniversitŠt Heidelberg.
Clontechniques April 1999