Gene trapping

from Strachan and Read, Human Molecular Genetics 2, Wiley-Liss, New York, 1999 (on reserve)

from Friedrich and Soriano Genes and Development 5:1513-1523 (1991)

Chimeric embryos containing promoter trap ES cells were stained with X-gal to reveal beta-galactosidase activity.

A, B. ROSAbeta-geoB, E8dpc

C. SAbeta-geo4, E13dpc

D. ROSAbeta-geo5, E12dpc

E. SAbeta-geo3, E12dpc

F. SAbeta-gal9, E12dpc

G. ROSAbeta-geo5, E8

H. ROSAbeta-geo9, E12

I. ROSAbeta-geo21, E9