Preclinical Imaging

SPECT-CT Imaging of Liver Metastases
SPECT-CT imaging of liver metastases

SPECT-CT imaging of liver metastases: Plectin-1 imaging allows noninvasive detection of liver metastases. AK134 cells, or saline (null), were injected intrasplenically to produce liver metastases. Left: mice bearing liver metastases (LM) from injection of Panc-1 (a pancreatic cancer cell line). Right: null animals without intrasplenic tumor cell injected. K=kidney. Images courtesy of Kim Kelly, PhD, biomedical Engineering, UVa.

Plec1 Imaging of Pancreatic Ductal Adenocarcinoma

In vivo imaging of Plec1 in orthotopic PDAC (pancreatic ductal adenocarcinoma). Mice bearing tumors from orthotopically implanted L3.6pl pancreatic tumor cells were injected with 111-In-tPTP (Plec1-targeting peptides) and imaged via SPECT/CT 4 hours postinjection. Note the accumulation of tPTP in PDAC, allowing the in vivo imaging of tumor in the pancreas and in peritoneal metastases. The 111-In-tPTP tracer was developed in the lab of Kim Kelly, PhD.

Maximum Intensity SPECT-CT Video

Click on the image above to see a movie of a maximum intensity projection of a SPECT-CT scan of a mouse bearing pancreatic tumors and associated peritoneal metastases, imaged using the Plectin1-targeting peptide tracer developed in the lab of Kim Kelly, PhD.
Slow Contrast Infusion System
Slow infusion system photo
Photograph of system designed for slow contrast infusion during microCT scanning. The rapid circulatory rate of mice makes a bolus injection of contrast impractical however this simple but effective infusion system, built in-house, permits a steady amount of contrast to be present in the vasculature for the ~5 minute microCT scans.
Cardiac Gated MicroCT With Slow Contrast Infusion

Images obtained using the in-house built slow fusion system (top row) and using the leading microCT vascular contrast agent, Fenestra VC (second row). Fenestra is designed to stay in the vasculature long enough for high quality contrast microCT scans. Prospective cardiac gating was used for all scans shown here. The three columns of images are, from left to right, axial, coronal, and sagittal. Note the superior contrast in the slow infusion images.

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SPECT CT scanner photo

We are developing instrumentation for preclinical imaging of animals such as rodents. The photograph to the left shows a preclinical scanner developed in our lab under NIH funding. It combines micro x-ray computed tomography (microCT) and micro single photon emission computed tomography (microSPECT) in an integrated scanner, permitting the animal to be scanned under anesthesia in the same configuration by both modalities. The SPECT and CT volumetric images are then fused prior to display.