Since we have spent a long time looking at the effects of reduced activation of the bulb, we began to look at the effects of sugically eliminating input to the bulb. By placing a small chip of Teflon between the bulb and the bone separating it from the nasal cavity (the cribriform plate) we can denervate a very small region of the bulb.
Below you see the effects of this procedure on the development of the bulb. The teflon chip is placed where the red line is. Changes in the organization in the bulb on the experimental side at this point (“a”) and at a control point (“b”) with age are shown in higher detail in the small panels at the bottom. P10 = postnatal day 10. Horizontal sections.
We have also looked at developmental changes in a host of other markers, including (from top to bottom ), olfactory marker protein, calretinin, calbindin, parvalbumin, map2, and tyrosine hydroxylase (in each panel at right, experimental tissue is on the top and control tissue is underneath)
Changes in cell morphology are also quite marked
Couper Leo, J. M., Devine A. M., and Brunjes P. C. Focal denervation alters cellular phenotypes and survival in the developing rat olfactory bulb. Journal of Comparative Neurology. 417, 325-336, 2000.
Couper Leo, J. M., Devine, A. M., and Brunjes P. C. Focal denervation alters cellular phenotypes and survival in the developing rat olfactory bulb: developmental analysis. J. Comp. Neurol. 425, 409-421, 2000.
Couper Leo, J. M., and Brunjes P. C. Neonatal focal denervation of the rat olfactory bulb alters cell structure and survival: a Golgi and Nissl and confocal study. Developmental Brain Research. 140, 277-286, 2003.