/95-12286%20H&E%20paraf.JPG)
/95-12286%20H&E%20froz.JPG)
/95-12286%20PAS%20without.JPG)
/95-12286%20PAS+amylase.JPG)
/95-12286%20phosphorylase.JPG)
/phosphorylase%20normal%20CTL.JPG)
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H&E stain on paraffin section Any myopathic features? Abnormal accumulations? |
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H&E stain on frozen section |
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Periodic Acid Schiff (PAS) stain for glycogen (also stains glycolipids and glycoproteins) Notice any abnormal accumulation? |
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Periodic Acid Schiff (PAS) stain for glycogen AFTER pretreatment with amylase to digest glycogen What does this stain tell you about the nature of the accumulated substance? |
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Phosphorylase (Myophosphorylase) histochemistry on PATIENT The lack of reaction is real |
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Phosphorylase (Myophosphorylase) histochemistry on a NORMAL CONTROL Activity is indicated by brown/purple deposit |
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