Lab Protocols:
Common filter sets and microscope light path configurations:
Wide-field light path illustrating the use of a multiband beam splitter: The path of light through the wide-field fluorescence microscope showing the position of the (1) arc lamp, (2) exciter filter wheel, (3) beamsplitter mirror, (4) obective, and (5) detector. The multi-band beamsplitter (see table above) has two regions of high transmission to accommodate the emission of both the donor and the acceptor fluorochromes (inset).
The light path configuration for the confocal microscope used for photoactivation of PA-GFP and for the detection of PQ-FRET.
Department of Medicine, PO Box 800578, University of Virginia Health System, Charlottesville, VA 22908-0578
Email: Phone: 434 982 3623