We are developing and using tools for the consistent and unbiased analysis of imaging data acquired from populations of living cells. For details see:

We are using the different color fluorescent proteins (FPs) as probes for protein localization and behavior in living cells. Through the expression of different proteins fuse to various color FPs, we can visualize the colocalization of proteins and their recruitment activities. For details see:

We are also using the technique of Förster resonance energy transfer (FRET) microscopy to define the spatial relationships of the coexpressed proteins. We have written several recent review articles and a book chapters describing this technique. For more information about the technique, follow the FRET Microscopy link below. For details about our research applications see:

The optical resolution of the light microscope is limited to about 200 nanometers (0.2 microns) and objects closer together than this will appear as a single object. The technique of FRET microscopy provides a method to define the spatial relationships of proteins labeled with fluorophores on a scale of less than 100 angstroms.

The following slide presentation provides a basic description of FRET microscopy, and describes the different methods used to measure FRET. Follow the link below :