Shook, D., C. Majer & R. Keller

Developmental Biology 270 (2004) 163-185. 

Movies are in Quicktime, which can be downloaded here.

Please contact David Shook at drs6j@virginia.edu if you have any problems downloading these files, or have any questions about them.

Figure S1. Confocal images on which Fig. 4B is based. Stage 22 X. laevis embryo, throuh a 40X lense, at 3 micrometer increments.

Figure S1 A: Rotation of projections of the confocal stack through 70 degrees by 10 degree increments, at a spacing of 8 pixels; the 7th frame is 180 degrees, which is the projection shown in Fig. 4B. The 2nd frame is that shown in the inset.

Figure S1 B: The confocal stack.

Figure S2. Timelapse movie showing the X. laevis dorsal isolate explant traced in Fig. 7. The blastopore is on the right. The movie begins at stage 13-14 and ends at stage 22. The movie shown here begins 3 hours and 42 minutes prior to the first frame shown in Fig. 7 (when the view jumps); frames are six minutes apart. The entire time covered by the movie is 8 hours and 57 mintues.

Figure S3. Timelapse movies showing two X. tropicalis dorsal isolate explants.

Figure S3 A: Blastopore is at the top. Frames are six minutes apart. The movie covers 7 hours and 24 minutes, from stage 12.5 to control stage 22-23. This dorsal isolate was pressed tightly under a cover slip.

Figure S3 B: The dorsal isolate on the left begins at about stage 14, the one on the right at about stage 13. Blastopores are at the top. The movie lasts 7 hours and 35 minutes, with frames 6 minutes apart. These dorsal isolates were pressed lightly under a coverslip. Movie ends at control stage 24. The jump in the middle of the movie occurs at control stage 18-19. Thus the lateral endodermal crests meet at the midline along most of the length of the trunk at about the same time the neural folds are meeting.