Supplementary Figures for:

Pattern and Morphogenesis of Presumptive Superficial Mesoderm in Two Closely Related Species, Xenopus laevis and X. tropicalis.

 

Shook, D., C. Majer & R. Keller

Developmental Biology 270 (2004) 163-185. 

 

Movies are in Quicktime, which can be downloaded here.

Please contact David Shook at drs6j@virginia.edu if you have any problems downloading these files, or have any questions about them.

 

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Figure S1. Confocal images on which Fig. 4B is based. Stage 22 X. laevis embryo, throuh a 40X lense, at 3 micrometer increments.

Figure S1 A: Rotation of projections of the confocal stack through 70 degrees by 10 degree increments, at a spacing of 8 pixels; the 7th frame is 180 degrees, which is the projection shown in Fig. 4B. The 2nd frame is that shown in the inset.

Figure S1 B: The confocal stack.

Figure S2. Timelapse movie showing the X. laevis dorsal isolate explant traced in Fig. 7. The blastopore is on the right. The movie begins at stage 13-14 and ends at stage 22. The movie shown here begins 3 hours and 42 minutes prior to the first frame shown in Fig. 7 (when the view jumps); frames are six minutes apart. The entire time covered by the movie is 8 hours and 57 mintues.

Figure S3. Timelapse movies showing two X. tropicalis dorsal isolate explants.

Figure S3 A: Blastopore is at the top. Frames are six minutes apart. The movie covers 7 hours and 24 minutes, from stage 12.5 to control stage 22-23. This dorsal isolate was pressed tightly under a cover slip.

Figure S3 B: The dorsal isolate on the left begins at about stage 14, the one on the right at about stage 13. Blastopores are at the top. The movie lasts 7 hours and 35 minutes, with frames 6 minutes apart. These dorsal isolates were pressed lightly under a coverslip. Movie ends at control stage 24. The jump in the middle of the movie occurs at control stage 18-19. Thus the lateral endodermal crests meet at the midline along most of the length of the trunk at about the same time the neural folds are meeting.