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Program

Sunday, May 12

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14:30-16:30	Lab column packing session (optional and included but must preregister to attend)
16:30-17:00	Registration
17:00-17:30	Introduction to the course
17:30-19:00	Lecture: Downstream processing and biophysical properties of biotechnology products
19:00-21:00	Reception

Monday, May 13

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8:30-10:30	Lecture: Introduction to Chromatography, basic principles, modes of operation
10:30-12:00	Lecture: Band broadening factors, plate model, scale-up relationships
12:00-13:00	Lunch
13:00-14:00	Lecture: The chromatographic workstation
14:00-16:00	Laboratory I: Isocratic elution, pulse response, determination of retention factor & HETP
16:00-17:30	Lecture: Categories and properties of chromatography media

Tuesday, May 14

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8:30-11:00	Evaluation and presentation of Laboratory I data
11:00-12:30	Lecture: Mass transfer effects - fundamentals
12:30-13:30	Lunch
13:30-15:30	Lecture: Mass transfer effects - applications
15:30-17:30	Laboratory II: Frontal analysis, dynamic binding capacity, scale-up parameters

Wednesday, May 15

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8:30-11:00	Evaluation and presentation of Laboratory II data
11:00-12:30	Lecture: Monoclonal antibody capture with Protein A adsorbents
12:30-13:30	Lunch
13:30-14:30	Lecture: Linear gradient elution theory and practice
14:30-17:30	Laboratory III: Gradient elution, determination of retention factor vs. salt

Thursday, May 16

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8:30-11:00	Evaluation and presentation of Laboratory III data
11:00-12:30	Lecture: Hydrophobic interaction chromatography, protein/surface and protein/protein interactions
12:30-13:30	Lunch
13:30-15:00	Lecture: Chromatographic process development and design
15:00-16:00	Lecture: Analytical technology to support process developemnt and operation
16:00-17:30	Laboratory IV: Gradient elution, determination of HETP and optimization of separation
18:00	Course dinner

Friday, May 17

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9:00-10:30	Evaluation and presentation of Laboratory IV data
10:30-12:30	Team exercises: Chromatography process design and optimization
12:30-13:30	Lunch