Short Course in

Protein Chromatography

Engineering Fundamentals and Measurements
for Process Development and Scale-up

May 13-18, 2018 - University of Virginia, Charlottesville, Virginia


Introduction Description Program Venue Registration Contact Reference text


Sunday, May 13

14:30-16:30 Lab column packing session (optional and included but must preregister to attend)
16:30-17:00 Registration
17:00-17:30 Introduction to the course
17:30-19:00 Lecture: Downstream processing and biophysical properties of biotechnology products
19:00-21:00 Reception

Monday, May 14

8:30-10:30 Lecture: Introduction to Chromatography, basic principles, modes of operation
10:30-12:00 Lecture: Band broadening factors, plate model, scale-up relationships
12:00-13:00 Lunch
13:00-14:00 Lecture: Chromatographic workstations and process systems
14:00-16:00 Laboratory I: Isocratic elution, pulse response, determination of retention factor & HETP
16:00-17:30 Lecture: Categories and properties of chromatography media

Tuesday, May 15

8:30-11:00 Evaluation and presentation of Laboratory I data
11:00-12:30 Lecture: Mass transfer effects - fundamentals
12:30-13:30 Lunch
13:30-15:30 Lecture: Mass transfer effects - applications
15:30-17:30 Laboratory II: Frontal analysis, dynamic binding capacity, scale-up parameters

Wednesday, May 16

8:30-11:00 Evaluation and presentation of Laboratory II data
11:00-12:30 Lecture: Antibody capture with Protein A media
12:30-13:30 Lunch
13:30-14:30 Lecture: Linear gradient elution theory and practice
14:30-17:30 Laboratory III: Gradient elution, determination of retention factor vs. salt

Thursday, May 17

8:30-11:00 Evaluation and presentation of Laboratory III data
11:00-12:30 Lecture: Hydrophobic interaction chromatography and biomolecular perspectives
12:30-13:30 Lunch
13:30-15:30 Lecture: Chromatographic column design and optimization for capture and resolution
15:30-17:00 Laboratory IV: Gradient elution, determination of HETP and optimization of separation
17:30 Course dinner

Friday, May 18

9:00-10:30 Evaluation and presentation of Laboratory IV data
10:30-12:30 Team exercise: Chromatography column design and optimization
12:30-13:30 Lunch