Biochemistry and Molecular Biology:

·        Membrane protein engineering

·        Membrane protein expression

·        Membrane protein purification

·        Membrane protein reconstitution with lipids

·        Activity measurements

 

Biophysical Techniques:

·        NMR spectroscopy

·        Site-directed spin-label EPR spectroscopy

·        Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy

·        Isothermal titration calorimetry (ITC)

·        Single molecule fluorescence imaging and tracking

·        Supported bilayer technology

·        Total internal reflection fluorescence microscopy (TIRFM)

·        Fluorescence recovery after photobleaching (FRAP)

·        Fluorescence interference contrast microscopy (FLIC)

 

Cell Biological Methods:

·        Mammalian cell culture

·        Expression of fluorescent and mutant proteins in mammalian cells

·        Fluorescence imaging and cell fusion assays

·        Immuno-precipatiation and FACS analysis