4A-4F. Wholemount in situ hybridization of S. tropicalis embryos with X. laevis probes.
4A. Engrailed 2 probe with both X. laevis (X.l.) and S. tropicalis (S.t.) neural tube stage embryos showing characteristic expression at midbrain/hindbrain boundary (arrow).
4B. Xbra expression in notochord (arrows) and posterior mesoderm (arrowhead) of late gastrula.
4C. Pax-6 expression in anterior neural (arrows) and presumptive spinal cord (arrowheads) of neural plate stage embryo.
4D. Six-3 expression in eye (arrow) of tailbud embryo.
4E. Sox-3 expression in neural tissue (arrows) of tailbud embryo.
4F. ?-crystallin expression in tadpole lens.
4G, 4H. Wholemount immunohistochemistry with antibodies against muscle-specific antigen 12-101 (G) and neural-specific antigen 6F11 (H). Arrows (H) show neural expression.