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June 18, 2008

Updated lab methodology for identifying conserved noncoding sequences in the
X. tropicalis
genome
("Genome Analysis" button at left)


New Updates
on Mutations and Genetic Technology

Lab methodology for in vivo ChIP Analysis in Xenopus embryos ("Genome Analysis" button at left)

New Transgenesis Protocol

The pipid frog Xenopus tropicalis presents the opportunity to combine sophisticated embryological methods with developmental genetics in a vertebrate system. The recently sequenced genome of X. tropicalis affords unique opportunities for genomic studies as well because its evolutionary distance from mammals is such that it is possible to identify long regions of synteny and conserved gene regulatory elements. New, high throughput methods for transgenesis allow one to examine the function of these regulatory elements and to rapidly prepare transgenic lines for a variety of purposes.

Closely resembling those of its well-characterized cousin, Xenopus laevis, X. tropicalis embryos are readily manipulated by techniques ranging from explant assays to transgenesis, and can be evaluated with most X. laevis molecular probes.

Unlike the slow-growing, tetraploid X. laevis, however, X. tropicalis is diploid and has a relatively short (<5 months) life cycle, greatly increasing the feasibility of multigeneration genetic analysis.

Our group, which includes collaborators across the United States and Europe, is continuing to develop X. tropicalis as a model organism. We are optimizing husbandry regimes to further shorten generation time, adapting X. laevis protocols, and assembling a set of genetic research tools including chemical and insertional mutagenesis, gene traps, stable transgenic reporter lines, inducible gene expression systems, and genetic and physical maps of the X. tropicalis genome.

This work is supported by NIH grant RR13221.

Principal Investigator
Robert Grainger, University of Virginia
Department of Biology, Gilmer Hall
PO Box 400328
Charlottesville, VA. 22904-4328
Phone: (434) 982-5495
Fax: (434) 982-5626
E-mail: rmg9p@virginia.edu

Other laboratories involved in the NIH genetics consortium:

Enrique Amaya, University of Manchester , U.K.  Website

Frank Conlon, University of North Carolina , Chapel Hill   Website

Richard Harland, University of California , Berkeley   Website

Mustafa Khokha, Yale University  Website

Paul Mead, St. Jude Children's Hospital   Website

Amy Sater, University of Houston   Website

Derek Stemple, Sanger Centre, U.K.   Website

Lyle Zimmerman, National Institute for Medical Research, U.K.  Website

 

 'Heartbreaker'



 'Arrested Development'



 'Bad Dog'



 'Rocinante'

For more information about this project please contact us.
Last update: Feb. 13, 2008.