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Cryoprotectant

 

We have tested a variety of cryoprotectants for their efficacy in preserving viable sperm. Our best results were achieved from the following formula:

  1. Separate the yolk of a chicken egg from the white. The yolk was then transferred into a 50 mL conical tube and diluted 1:1 with sterile water. (An egg yolk from a large egg is approximately 15mL). The contents were lightly vortexed to ensure homogenization.

  2. The diluted egg yolk was then diluted 20% (v:v) in a solution consisting of 0.4 M sucrose and 20 mM sodium bicarbonate (sterile filtered).

  3. The solution was then centrifuged at 13,000 rpm in a refrigerated centrifuge and the supernatant aliquotted as the cryoprotectant. Aliquots were stored at 80oC and thawed on ice when needed for use.

 

 

For more information about this project please contact us.
Last update: Feb. 13, 2008