Our lab is studying developmental defects and induced mutations in Xenopus tropicalis to better understand the different aspects of gene function and regulation. Our project has involved two phases thus far: screening of wild caught X. tropicalis frogs for naturally-occurring mutations and screening of the F1 generation of ENU-mutagenized males (F0) for mutations induced chemically.
The screening is ongoing, but the lab hopes to begin characterizing some of the mutations with the new genetic resources available for X. tropicalis, such as the Xenopus tropicalis linkage map (http://tropmap.biology.uh.edu/) and the X. tropicalis genome map (http://genome.jgi-psf.org/Xentr4/Xentr4.home.html) . We also foresee offering some of our mutants to the Xenopus community for study since many fall outside the scope of our knowledge of various systems and processes.
With new technology developed in the Grainger lab, screening animals for mutations has become easier than ever. Our method now provides a low cost, high throughput technique that will allow smaller labs to screen animals that would have been precluded by the previous methods due to fiscal or space limitations.
How to set up a large screen of X. tropicalis frogs
Screening X. tropicalis using Gynogenesis
Screening males for mutations using frozen sperm and TILLING
Naturally occurring mutations
Mutations from ENU F1 animals